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02501 primescripttm reverse transcriptase takara  (TaKaRa)


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    Structured Review

    TaKaRa 02501 primescripttm reverse transcriptase takara
    02501 Primescripttm Reverse Transcriptase Takara, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 88241 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/takara+primescript+reverse+transcriptase/PrimeScript+RT+Reagent+Kit/pm41997144-265-110-114
    Average 99 stars, based on 88241 article reviews
    02501 primescripttm reverse transcriptase takara - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Rapamycin Inhibits Osteoclastogenesis and Prevents LPS-Induced Alveolar Bone Loss by Oxidative Stress Suppression
    Article Snippet: .. Total RNA was extracted from BMMs and RAW 264.7 cells after OC formation using a Trizol kit, following a standard protocol. cDNA was obtained through reverse transcription from an aliquot of 500 ng of RNA, by TaKaRa PrimeScript Reverse Transcriptase (TaKaRa Bio Inc., Shiga, Japan). .. Quantitative PCR was performed using the SYBR green mixture (Takara) to detect the expression of genes.

    Article Title: The BAHD acyltransferase STBR1 confers both saline-alkali tolerance and blast resistance by stabilizing the non-canonical catalase CATA to promote H 2 O 2 scavenging in rice
    Article Snippet: .. Total RNA was isolated from leaves using the TIANGEN RNAprep Pure Plant Kit (DP432), and cDNA synthesis was performed using the TaKaRa PrimeScript reverse transcriptase for quantitative PCR. .. Reactions were carried out with the TaKaRa SYBR Premix Ex Taq kit on the Bio-Rad CFX96 Real-Time System.

    Article Title: Odontoblasts release exosomes to regulate the odontoblastic differentiation of dental pulp stem cells.
    Article Snippet: Briefly, TRIzol (Takara, Shiga, Japan) was used to extract the total RNA from the cells according to the manufacturer’s instructions. .. An aliquot of 1000 ng of RNA was reverse transcribed into cDNA using TaKaRa PrimeScript Reverse Transcriptase (TaKaRa, Shiga, Japan). qRT‒ PCR was performed with SYBR Premix DimerEraserTM (Takara) using a QuantStudio 5 Real-time PCR system (Applied Biosystems, USA). ..

    Article Title: Coronavirus shedding in New Zealand bats: insights and future perspectives
    Article Snippet: .. Then RNA was extracted following the manufacturer’s protocol and eventually resuspended in 40 μL RNAse-free water. cDNAs were obtained using Takara Primescript Reverse Transcriptase, oligo (dT) primer and random 6 mers (Takara, Kusatsu, Japan) following the manufacturer’s protocol. ..

    Article Title: Chitosan/dsRNA polyplex nanoparticles advance environmental RNA interference efficiency through activating clathrin-dependent endocytosis.
    Article Snippet: Chitosan, as a promising gene nanocarrier for enhancing RNA interference (RNAi) efficiency, displays tremendous application prospects in addressing dsRNA delivery concerns.. However, the molecular mechanism of chitosan/dsRNA polyplex nanoparticles (PNs) for advancing dsRNA delivery efficiency remains largely unknown.. Here, chitosan/dsRNA PNs were prepared by an electrostatic attraction method.

    Article Title: Odontoblasts release exosomes to regulate the odontoblastic differentiation of dental pulp stem cells
    Article Snippet: Briefly, TRIzol (Takara, Shiga, Japan) was used to extract the total RNA from the cells according to the manufacturer’s instructions. .. An aliquot of 1000 ng of RNA was reverse transcribed into cDNA using TaKaRa PrimeScript Reverse Transcriptase (TaKaRa, Shiga, Japan). qRT‒PCR was performed with SYBR Premix DimerEraserTM (Takara) using a QuantStudio 5 Real-time PCR system (Applied Biosystems, USA). ..

    Article Title: Increased neuron density in the midbrain of a foveate bird, pigeon, results from profound change in tissue morphogenesis.
    Article Snippet: RNA quantification was done with spectrophotometer and Qubit 2.0 (ThermoFisher) and RNA quality was checked using BioAnalyzer 2100 (Agilent). .. RT was performed using Takara PrimeScript reverse transcriptase prior to qPCR. ..

    Article Title: Rapamycin Inhibits Osteoclastogenesis and Prevents LPS-Induced Alveolar Bone Loss by Oxidative Stress Suppression.
    Article Snippet: .. Total RNA was extracted from BMMs and RAW 264.7 cells after OC formation using a Trizol kit, following a standard protocol. cDNA was obtained through reverse transcription from an aliquot of 500 ng of RNA, by TaKaRa PrimeScript Reverse Transcriptase (TaKaRa Bio Inc., Shiga, Japan). .. Quantitative PCR was performed using the SYBR green mixture (Takara) to detect the expression of genes.

    Isolation:

    Article Title: The BAHD acyltransferase STBR1 confers both saline-alkali tolerance and blast resistance by stabilizing the non-canonical catalase CATA to promote H 2 O 2 scavenging in rice
    Article Snippet: .. Total RNA was isolated from leaves using the TIANGEN RNAprep Pure Plant Kit (DP432), and cDNA synthesis was performed using the TaKaRa PrimeScript reverse transcriptase for quantitative PCR. .. Reactions were carried out with the TaKaRa SYBR Premix Ex Taq kit on the Bio-Rad CFX96 Real-Time System.

    cDNA Synthesis:

    Article Title: The BAHD acyltransferase STBR1 confers both saline-alkali tolerance and blast resistance by stabilizing the non-canonical catalase CATA to promote H 2 O 2 scavenging in rice
    Article Snippet: .. Total RNA was isolated from leaves using the TIANGEN RNAprep Pure Plant Kit (DP432), and cDNA synthesis was performed using the TaKaRa PrimeScript reverse transcriptase for quantitative PCR. .. Reactions were carried out with the TaKaRa SYBR Premix Ex Taq kit on the Bio-Rad CFX96 Real-Time System.

    Real-time Polymerase Chain Reaction:

    Article Title: The BAHD acyltransferase STBR1 confers both saline-alkali tolerance and blast resistance by stabilizing the non-canonical catalase CATA to promote H 2 O 2 scavenging in rice
    Article Snippet: .. Total RNA was isolated from leaves using the TIANGEN RNAprep Pure Plant Kit (DP432), and cDNA synthesis was performed using the TaKaRa PrimeScript reverse transcriptase for quantitative PCR. .. Reactions were carried out with the TaKaRa SYBR Premix Ex Taq kit on the Bio-Rad CFX96 Real-Time System.

    Article Title: Odontoblasts release exosomes to regulate the odontoblastic differentiation of dental pulp stem cells.
    Article Snippet: Briefly, TRIzol (Takara, Shiga, Japan) was used to extract the total RNA from the cells according to the manufacturer’s instructions. .. An aliquot of 1000 ng of RNA was reverse transcribed into cDNA using TaKaRa PrimeScript Reverse Transcriptase (TaKaRa, Shiga, Japan). qRT‒ PCR was performed with SYBR Premix DimerEraserTM (Takara) using a QuantStudio 5 Real-time PCR system (Applied Biosystems, USA). ..

    Article Title: Odontoblasts release exosomes to regulate the odontoblastic differentiation of dental pulp stem cells
    Article Snippet: Briefly, TRIzol (Takara, Shiga, Japan) was used to extract the total RNA from the cells according to the manufacturer’s instructions. .. An aliquot of 1000 ng of RNA was reverse transcribed into cDNA using TaKaRa PrimeScript Reverse Transcriptase (TaKaRa, Shiga, Japan). qRT‒PCR was performed with SYBR Premix DimerEraserTM (Takara) using a QuantStudio 5 Real-time PCR system (Applied Biosystems, USA). ..

    Article Title: Increased neuron density in the midbrain of a foveate bird, pigeon, results from profound change in tissue morphogenesis.
    Article Snippet: RNA quantification was done with spectrophotometer and Qubit 2.0 (ThermoFisher) and RNA quality was checked using BioAnalyzer 2100 (Agilent). .. RT was performed using Takara PrimeScript reverse transcriptase prior to qPCR. ..

    Polymerase Chain Reaction:

    Article Title: Odontoblasts release exosomes to regulate the odontoblastic differentiation of dental pulp stem cells.
    Article Snippet: Briefly, TRIzol (Takara, Shiga, Japan) was used to extract the total RNA from the cells according to the manufacturer’s instructions. .. An aliquot of 1000 ng of RNA was reverse transcribed into cDNA using TaKaRa PrimeScript Reverse Transcriptase (TaKaRa, Shiga, Japan). qRT‒ PCR was performed with SYBR Premix DimerEraserTM (Takara) using a QuantStudio 5 Real-time PCR system (Applied Biosystems, USA). ..

    In Vitro:

    Article Title: Chitosan/dsRNA polyplex nanoparticles advance environmental RNA interference efficiency through activating clathrin-dependent endocytosis.
    Article Snippet: Chitosan, as a promising gene nanocarrier for enhancing RNA interference (RNAi) efficiency, displays tremendous application prospects in addressing dsRNA delivery concerns.. However, the molecular mechanism of chitosan/dsRNA polyplex nanoparticles (PNs) for advancing dsRNA delivery efficiency remains largely unknown.. Here, chitosan/dsRNA PNs were prepared by an electrostatic attraction method.



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